Site-directed hydroxy radical mapping of nucleosome positions in vitro (PROT21)
نویسندگان
چکیده
The dynamics of chromatin structure is becoming an area of increasing interest. Both thermal energy and ATP-dependent chromatin remodelling enzymes can alter nucleosome structure and positioning. In addition, modifications to both the DNA and histones and changes in their composition can influence the rate of these changes. The ability to precisely determine the position of a nucleosome in vitro is extremely useful when studying the biochemical behaviour of chromatin and its modifying enzymes.
منابع مشابه
Parallel mapping with site-directed hydroxyl radicals and micrococcal nuclease reveals structural features of positioned nucleosomes in vivo
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